An assessment of AVIRIS data for hydrothermal alteration mapping in the Goldfield Mining District, Nevada

Airborne Visible and Infrared Imaging Spectrometer (AVIRIS) data were acquired over the Goldfield Mining District, Nevada, in September 1987. Goldfield is one of the group of large epithermal precious metal deposits in Tertiary volcanic rocks, associated with silicic volcanism and caldera formation. Hydrothermal alteration consists of silicification along fractures, advanced agrillic and argillic zones further away from veins and more widespread propylitic zones. An evaluation of AVIRIS data quality was performed. Faults in the data, related to engineering problems and a different behavior of the instrument while on-board the U2, were encountered. Consequently, a decision was made to use raw data and correct them only for dark current variations and detector read-out-delays. New software was written to that effect. Atmospheric correction was performed using the flat field correction technique. Analysis of the data was then performed to extract spectral information, mainly concentrating on the 2 to 2.45 micron window, as the alteration minerals of interest have their distinctive spectral reflectance features in this region. Principally kaolinite and alunite spectra were clearly obtained. Mapping of the different minerals and alteration zones was attempted using ratios and clustering techniques. Poor signal-to-noise performance of the instrument and the lack of appropriate software prevented the production of an alteration map of the area. Spectra extracted locally from the AVIRIS data were checked in the field by collecting representative samples of the outcrops

Symmetrization in jellyfish: reorganization to regain function, and not lost parts

We recently reported a previously unidentified strategy of self-repair in the moon jellyfish Aurelia aurita. Rather than regenerating lost parts, juvenile Aurelia reorganize remaining parts to regain essential body symmetry. This process that we called symmetrization is rapid and frequent, and is not driven by cell proliferation or cell death. Instead, the swimming machinery generates mechanical forces that drive symmetrization. We found evidence for symmetrization across three other species of jellyfish (Chrysaora pacifica, Mastigias sp., and Cotylorhiza tuberculata). We propose reorganization to regain function without recovery of initial morphology as a potentially broad class of self-repair strategy beyond radially symmetrical animals, and discuss the implications of this finding on the evolution of self-repair strategies in animals

Upside-Down but Headed in the Right Direction: Review of the Highly Versatile Cassiopea xamachana System

The upside-down jellyfish Cassiopea xamachana (Scyphozoa: Rhizostomeae) has been predominantly studied to understand its interaction with the endosymbiotic dinoflagellate algae Symbiodinium. As an easily culturable and tractable cnidarian model, it is an attractive alternative to stony corals to understanding the mechanisms driving establishment and maintenance of symbiosis. Cassiopea is also unique in requiring the symbiont in order to complete its transition to the adult stage, thereby providing an excellent model to understand symbiosis-driven development and evolution. Recently, the Cassiopea research system has gained interest beyond symbiosis in fields related to embryology, climate ecology, behavior, and more. With these developments, resources including genomes, transcriptomes, and laboratory protocols are steadily increasing. This review provides an overview of the broad range of interdisciplinary research that has utilized the Cassiopea model and highlights the advantages of using the model for future research

Site Characterization Using Integrated Imaging Analysis Methods on Satellite Data of the Islamabad, Pakistan, Region

We develop an integrated digital imaging analysis approach to produce a first-approximation site characterization map for Islamabad, Pakistan, based on remote-sensing data. We apply both pixel-based and object-oriented digital imaging analysis methods to characterize detailed (1:50,000) geomorphology and geology from Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) satellite imagery. We use stereo-correlated relative digital elevation models (rDEMs) derived from ASTER data, as well as spectra in the visible near-infrared (VNIR) to thermal infrared (TIR) domains. The resulting geomorphic units in the study area are classified as mountain (including the Margala Hills and the Khairi Murat Ridge), piedmont, and basin terrain units. The local geologic units are classified as limestone in the Margala Hills and the Khairi Murat Ridge and sandstone rock types for the piedmonts and basins. Shear-wave velocities for these units are assigned in ranges based on established correlations in California. These ranges include Vs30-values to be greater than 500 m/sec for mountain units, 200–600 m/sec for piedmont units, and less than 300 m/sec for basin units. While the resulting map provides the basis for incorporating site response in an assessment of seismic hazard for Islamabad, it also demonstrates the potential use of remote-sensing data for site characterization in regions where only limited conventional mapping has been done

3D-printable tools for developmental biology: Improving embryo injection and screening techniques through 3D-printing technology

Developmental biology requires rapid embryo injections and screening. We applied new affordable high-resolution 3D-printing to create five easily modifiable stamp-mold tools that greatly increase injection and screening speed, while simultaneously reducing the harmful aspects of these processes. We designed two stamps that use different approaches to improve the injection efficiency for two different types of embryo, first for embryos from the snail Crepidula fornicata, and second, for those from the spider Parasteatoda tepidariorum. Both drastically improved injection speeds and embryo survival rates, even in novice hands. The other three tools were designed for rapid side-by-side organism orientating and comparison. The first screening tool allows for optimal imaging in Xenopus laevis tadpoles, while the second and third facilitate rapid high-throughput screening of Xenopus tropicalis tadpoles and Danio rerio juveniles, respectively. These designs can act as templates for many injection or screening applications

Uplift and subsidence associated with the great Aceh-Andaman earthquake of 2004

Rupture of the Sunda megathrust on 26 December 2004 produced broad regions of uplift and subsidence. We define the pivot line separating these regions as a first step in defining the lateral extent and the downdip limit of rupture during that great M_w ≈ 9.2 earthquake. In the region of the Andaman and Nicobar islands we rely exclusively on the interpretation of satellite imagery and a tidal model. At the southern limit of the great rupture we rely principally on field measurements of emerged coral microatolls. Uplift extends from the middle of Simeulue Island, Sumatra, at ~2.5°N, to Preparis Island, Myanmar (Burma), at ~14.9°N. Thus the rupture is ~1600 km long. The distance from the pivot line to the trench varies appreciably. The northern and western Andaman Islands rose, whereas the southern and eastern portion of the islands subsided. The Nicobar Islands and the west coast of Aceh province, Sumatra, subsided. Tilt at the southern end of the rupture is steep; the distance from 1.5 m of uplift to the pivot line is just 60 km. Our method of using satellite imagery to recognize changes in elevation relative to sea surface height and of using a tidal model to place quantitative bounds on coseismic uplift or subsidence is a novel approach that can be adapted to other forms of remote sensing and can be applied to other subduction zones in tropical regions

Self-repairing symmetry in jellyfish through mechanically driven reorganization

What happens when an animal is injured and loses important structures? Some animals simply heal the wound, whereas others are able to regenerate lost parts. In this study, we report a previously unidentified strategy of self-repair, where moon jellyfish respond to injuries by reorganizing existing parts, and rebuilding essential body symmetry, without regenerating what is lost. Specifically, in response to arm amputation, the young jellyfish of Aurelia aurita rearrange their remaining arms, recenter their manubria, and rebuild their muscular networks, all completed within 12 hours to 4 days. We call this process symmetrization. We find that symmetrization is not driven by external cues, cell proliferation, cell death, and proceeded even when foreign arms were grafted on. Instead, we find that forces generated by the muscular network are essential. Inhibiting pulsation using muscle relaxants completely, and reversibly, blocked symmetrization. Furthermore, we observed that decreasing pulse frequency using muscle relaxants slowed symmetrization, whereas increasing pulse frequency by lowering the magnesium concentration in seawater accelerated symmetrization. A mathematical model that describes the compressive forces from the muscle contraction, within the context of the elastic response from the mesoglea and the ephyra geometry, can recapitulate the recovery of global symmetry. Thus, self-repair in Aurelia proceeds through the reorganization of existing parts, and is driven by forces generated by its own propulsion machinery. We find evidence for symmetrization across species of jellyfish (Chrysaora pacifica, Mastigias sp., and Cotylorhiza tuberculata)

Upside-Down but Headed in the Right Direction: Review of the Highly Versatile Cassiopea xamachana System

The upside-down jellyfish Cassiopea xamachana (Scyphozoa: Rhizostomeae) has been predominantly studied to understand its interaction with the endosymbiotic dinoflagellate algae Symbiodinium. As an easily culturable and tractable cnidarian model, it is an attractive alternative to stony corals to understanding the mechanisms driving establishment and maintenance of symbiosis. Cassiopea is also unique in requiring the symbiont in order to complete its transition to the adult stage, thereby providing an excellent model to understand symbiosis-driven development and evolution. Recently, the Cassiopea research system has gained interest beyond symbiosis in fields related to embryology, climate ecology, behavior, and more. With these developments, resources including genomes, transcriptomes, and laboratory protocols are steadily increasing. This review provides an overview of the broad range of interdisciplinary research that has utilized the Cassiopea model and highlights the advantages of using the model for future research

Invertebrate Diet of Breeding and Nonbreeding Crested Caracaras (Caracara cheriway) in Florida

We compared the invertebrate component of the diet of breeding and nonbreeding northern Crested Caracaras (Caracara cheriway) during the breeding season, January through April, in Florida, using pellet analysis. Pellets from breeding adults were collected at active nests and pellets of nonbreeding caracaras were collected from beneath a communal roost. During the breeding season, breeding and nonbreeding caracaras consumed invertebrate prey from a minimum of 61 genera and a total of 33 families from eight orders. Nonbreedingcaracaras consumed greater total numbers of invertebrates, more different prey types, and a greater diversity of invertebrates than did breeding caracaras. Pellets of nonbreeding caracaras contained more invertebrates per pellet, and carrion insects occurred more frequently in pellets of nonbreeding caracaras. Our findings suggest hypotheses regarding the possible role of intraspecific competition in the use of particular habitats and food resources by breeding and nonbreeding caracaras in Florida. Breeding pairs of caracaras are highly territorial and primarily feed nestlings larger-sized vertebrate prey, and field observations indicated that territorial adults can exclude young (nonbreeding) birds from their breeding areas; thus, diet differences may reflect social structure within this population. If pairs of breeding adults exclude conspecifics from important food resources or foraging habitats, nonbreeders may hunt arthropods or rely on carrion by necessity, especially if nonbreeders occur in suboptimal habitat in which other prey are less available

Deterioration of western redcedar (Thuja plicata Donn ex D. Don) seeds: protein oxidation and in vivo NMR monitoring of storage oils

Deterioration of conifer seeds during prolonged storage has a negative impact on reforestation and gene conservation efforts. Western redcedar (Thuja plicata Donn ex D. Don) is a species of tremendous value to the forest industry. The seeds of this species are particularly prone to viability losses during long-term storage. Reliable tools to assess losses in seed viability during storage and their underlying causes, as well as the development of methods to prevent storage-related deterioration of seeds are needed by the forest industry. In this work, various imaging methods and biochemical analyses were applied to study deterioration of western redcedar seeds. Seedlots that exhibited poor germination performance, i.e. those that had experienced the greatest losses of viability during prolonged storage, exhibited greater abundance of oxidized proteins, detected by protein oxidation assays, and more pronounced changes in their in vivo (13)C NMR spectra, most likely due to storage oil oxidation. The proportion of oxidized proteins also increased when seeds were subjected to accelerated ageing treatments. Detection of oxidized oils and proteins may constitute a reliable and useful tool for the forest industry